- 鸡胚提取物
- 鸡胚提取物
产品描述 描述 鸡胚提取物是从生长9-12天的鸡胚中提取的,用于组织培养中的生长因子补充成分。
它是一种优质蛋白质,蛋白质含量高,氨基酸模式与人体的氨基酸模式一致,具有丰富的矿物元素和维生素,具有很高的营养价值。它的K、Fe、Se矿物元素和维生素得A、D、B2等含量也很高。这使得它不但具有补充这些营养素的作用,而且,还可能有一些保健功能。已有实验证明,鸡胚提取物能有效地改善小鼠的硒营养不良状态,提高低硒小鼠的GPX活性,清除过氧化物产物MDA。有文献提出它还具有调节血脂的功能。
该产品制备经无菌过滤(0.2μm),以10毫升冻干小瓶的形式提供。请注意,冻干产品无法保证无菌,重构后应通过无菌一次性过滤器重新过滤。
The product is prepared in conformance with cGMP.The proprietary process utilises 9-12 day eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.
It is processed as a 50% w/v extract in Dulbecco's Phosphate Buffered Saline. 100IU/ml Penicillin,100μg/ml Streptomycin and 2.5μg/ml Amphotericin are added as an additional safeguard.
Statistical samples representative of the batch are tested in agreement with cGLP to validate the product as being consistent with the designed quality standards of reliability and stability.
Typically used at a concentration of 0.1% to 2% in cell culture.应用 The product is intended for in-vitro cell culture only. 储存/保存方法 Recommended storage is -20°C or below. Lyophilized product may be stored at 2-8°C. 产品用途 Directions for Use
1、Thaw the product overnight at 4°C and then bring to room temperature.
2、Use a 0.2um filtered ultra-purified water, and add to vials using a 10mL syringe with a hypodermic needle. Allow water to be draw in by vacuum.
3、Place samples on their sides and mix using an orbital shaker set at 150rpm at room temperature for 30 minutes.
4、To filter, use sterile gauze if necessary, then a 0.45um filter, then finally use a 0.2um filter.基本信息 别名 CEE,Chicken Embryo Extract 可溶性/溶解性 Reconstitute with water to 50% w/v. Solutions can be filtered through a 0.2 μm sterile filter as sterile. 参考文献 参考文献 1. Yablonka-Reuveni, Z., "Myogenesis in the chicken: The onset of differentiation of adultmyoblasts is influenced by tissue factors." Basic and Applied Myology, v. 5(1), 33 (1995).
2. Beurg, M., et al., "Differential regulation of skeletal muscle L-Type Ca2+ current and excitation
-contraction coupling by the dihydropyridine receptor beta subunit." BiophysicalJournal, v. 76, 1744-1756 (1999).温馨提示:本产品仅作科研实验使用,不支持临床等研究