- 鸡胚提取物
- 鸡胚提取物
产品描述 描述 鸡胚提取物是从生长9-12天的鸡胚中提取的，用于组织培养中的生长因子补充成分。
鸡胚提取物是一种优质蛋白质，蛋白质含量高，氨基酸模式与人体的氨基酸模式一致，具有丰富的矿物元素和维生素，具有很高的营养价值。它的K、Fe、Se矿物元素和维生suA、D、B2等含量也很高。这使得它不但具有补充这些营养素的作用，而且，还可能有一些保健功能。已有实验证明，鸡胚提取物能有效地改善小鼠的硒营养不良状态，提高低硒小鼠的GPX活性，清除过氧化物产物MDA。有文献提出鸡胚提取物还具有调节血脂的功能。
在磷酸盐缓冲盐水中以50%的提取物进行处理，补充100IU/mL青霉su、100ug/mL链霉su和2.5ug/mL两性霉素。将液体提取物无菌过滤至0.2µm，并在10mL等分试样中冻干。这种冻干粉的无菌性无法保证，应在重组后再次进行无菌过滤。
The product is prepared in conformance with cGMP.The proprietary process utilises 9-12 day eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.
It is processed as a 50% w/v extract in Dulbecco's Phosphate Buffered Saline. 100IU/ml Penicillin，100μg/ml Streptomycin and 2.5μg/ml Amphotericin are added as an additional safeguard.
Statistical samples representative of the batch are tested in agreement with cGLP to validate the product as being consistent with the designed quality standards of reliability and stability.
Typically used at a concentration of 0.1% to 2% in cell culture.应用 The product is intended for in-vitro cell culture only. 储存/保存方法 Recommended storage is -20°C or below. Lyophilized product may be stored at 2-8°C. 产品用途 Directions for Use
1、Thaw the product overnight at 4°C and then bring to room temperature.
2、Use a 0.2um filtered ultra-purified water, and add to vials using a 10mL syringe with a hypodermic needle. Allow water to be draw in by vacuum.
3、Place samples on their sides and mix using an orbital shaker set at 150rpm at room temperature for 30 minutes.
4、To filter, use sterile gauze if necessary, then a 0.45um filter, then finally use a 0.2um filter.注意事项 用10毫升无菌组织培养级水重新配制冻干产品。搅拌混合。如果出现沉淀物，在冷却条件下继续搅拌数小时/一夜或超声处理，简要使用超声波水浴。然后，在3000 x g下离心10分钟，并移除颗粒。之后重组冻干产品必须通过0.2µm过滤器过滤，以保持无菌。复原后，在4°C下储存以供立即使用，或等份加入工作体积，并在-20至-10°C长期储存，最大限度地减少冻融循环解冻时可观察到蛋白质沉淀。为了消除这种情况，可以在3000×g的条件下离心10分钟。 基本信息 别名 鸡胚提取液;CEE；Chicken Embryo Extract 可溶性/溶解性 Reconstitute with water to 50% w/v. Solutions can be filtered through a 0.2 μm sterile filter as sterile. 参考文献 参考文献 1. Yablonka-Reuveni, Z., "Myogenesis in the chicken: The onset of differentiation of adultmyoblasts is influenced by tissue factors." Basic and Applied Myology, v. 5(1), 33 (1995).
2. Beurg, M., et al., "Differential regulation of skeletal muscle L-Type Ca2+ current and excitation
-contraction coupling by the dihydropyridine receptor beta subunit." BiophysicalJournal, v. 76, 1744-1756 (1999).温馨提示：本产品仅作科研实验使用，不支持临床等研究