| 概述 |
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| 别名 | Rabbit Anti-VEGF Polyclonal Antibody VEGF抗体;Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor. |
| 宿主 | Rabbit |
| 特异性 | VEGF |
| 反应种属 | Human; |
| 预测反应种属 | Dog;Pig;Cow;Horse;Rabbit;Sheep; |
| 应用 | Rabbit Anti-VEGF Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） |
| 免疫原 | KLH conjugated synthetic peptide derived from human VEGF:81-132/232 |
| 性能 |
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| 浓度 | 1mg/ml |
| 纯化方法 | affinity purified by Protein A |
| 类型 | Polyclonal Antibody |
| 同种型 | IgG |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 生长因子和激素 血管内皮细胞 |
| 靶标 |
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| 背景说明 | This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq]. |
| 细胞定位 | 细胞外基质 分泌型蛋白
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| UniProt | P15692 |
| 实验结果图 |
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| Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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