Rabbit Anti-IL-1 Beta Polyclonal Antibodyabs120224－爱必信（上海）生物科技有限公司

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Rabbit Anti-IL-1 Beta Polyclonal Antibody
Rabbit Anti-IL-1 Beta Polyclonal Antibody

概述
别名	Rabbit Anti-IL-1 Beta Polyclonal AntibodyIL-1 β抗体;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. IL-1B
宿主	Rabbit
特异性	IL-1 Beta
反应种属	Human;Mouse;Rat;
预测反应种属	Dog;Pig;Rabbit;Guinea Pig;
应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
免疫原	KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269
性能
浓度	1mg/ml
纯化方法	affinity purified by Protein A
类型	Polyclonal Antibody
同种型	IgG
储存/保存方法	Rabbit Anti-IL-1 Beta Polyclonal Antibody Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
存储溶液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
研究领域	细胞生物免疫学糖蛋白
靶标
背景说明	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
细胞定位	分泌型蛋白
UniProt	P01584
实验结果图
	Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug Primary: Anti- IL-1 beta (abs120224) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 30 kD
	Tissue/cell: Human cervical carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Blank control (black line): A431(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary antibody: Rabbit Anti-PTP1B antibody ;Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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