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- Recombinant Rat IFN-γ
- Recombinant Rat IFN-γ
概述 种属 Rat 形态 Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80. 来源 Escherichia coli. 氨基酸序列 QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC 内毒素水平 Less than 0.1 EU/µg of rRtIFN-γ as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Accession # P01581 预期分子量 Approximay 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 97 % by SDS-PAGE and HPLC analyses. Gene IDs 25712 参考文献 1. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>2. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.<br/>3. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>4. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.