IFN-γ蛋白

Murine

Escherichia coli.

Lyophilized from a 0.2 um filtered solution in 20 mM Tris-HCI pH8.0, 300 mM NaCl, containing 5 % trehalose, 0.05 % Tween-20.（于2022.12.16更新）

HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC

No Tag

> 96 % by SDS-PAGE and HPLC analyses.

Less than 1 EU/μg of rMuIFN-γ as determined by LAL method.

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 106 IU/mg.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled 20 mM Tris-HCl pH8.0 with 50 mM NaCl to a concentration of 0.1-0.3 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Avoid repeated freeze/thaw cycles.

P01580

15978

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4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.