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- Recombinant Human IFN-ω
- Recombinant Human IFN-ω
概述 种属 Human 形态 Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. 来源 Escherichia coli. 氨基酸序列 CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LIFSLFHT ERSSAAWNMT LLDQLHTGLH LQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS 内毒素水平 Less than 1 EU/μg of Recombinant HumanIFN-ω as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg. Accession # P05000 预期分子量 Approximay 20.0 kDa, containing 172 amino acid residues with two conserved disulfide bonds. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 97 % by SDS-PAGE and HPLC analyses. Gene IDs 3467 参考文献 1. Yang LM, Xue QH, Sun L, et al. 2007. J Interferon Cytokine Res, 27: 119-27.<br/>2. Liu L, Leaman DW, Bixby JA, et al. 1996. Biochim Biophys Acta, 1294: 55-62.<br/>3. Adolf GR. 1990. Virology, 175: 410-7.<br/>4. Benoit P, Maguire D, Plavec I, et al. 1993. J Immunol, 150: 707-16.