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- Recombinant Human M-CSF（10ug）
- Recombinant Human M-CSF（10ug）
概述 种属 Human 形态 Lyophilized from a 0.2 μm filtered solution in PBS, pH7.4. 来源 Escherichia coli. 氨基酸序列 EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS 内毒素水平 Less than 1 EU/μg of Recombinant HumanM-CSF as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. Accession # P09603-3 预期分子量 Approximay 36.8 kDa, a disulfide-linked homodimer consisting of two 158 amino acid polypeptide chains. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 95 % by SDS-PAGE and HPLC analyses. Gene IDs 1435 参考文献 1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.<br/>2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.<br/>3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.<br/>4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.