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- Recombinant Human G-CSF(2ug)
- Recombinant Human G-CSF(2ug)
概述 种属 Human 形态 Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0. 来源 Escherichia coli. 氨基酸序列 TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIW MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP 内毒素水平 Less than 1 EU/μg of Recombinant HumanG-CSF as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Accession # P09919 预期分子量 Approximay 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 98 % by SDS-PAGE and HPLC analyses. Gene IDs 1440 参考文献 1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.<br/>2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.<br/>3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.<br/>4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.<br/>5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.