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- Recombinant Canine GM-CSF（5ug）
- Recombinant Canine GM-CSF（5ug）
概述 种属 Canine 形态 Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. 来源 Escherichia coli. 氨基酸序列 APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK 内毒素水平 Less than 1 EU/μg of rCaGM-CSF as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg. Accession # P48749 预期分子量 Approximay 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 95 % by SDS-PAGE and HPLC analyses. Gene IDs 403923 参考文献 1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.<br/>2. 1989. N Engl J Med, 320: 253-4.<br/>3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.<br/>4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.<br/>5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.