- Donkey anti-Mouse IgG-Cy3
- Donkey anti-Mouse IgG-Cy3
概述 描述 Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
克隆类别 Polyclonal 同种亚型 Whole IgG 反应种属 Mouse 来源宿主 Donkey 形态 Liquid 特异性 IgG (H+L) 使用方法
Histo-/Cyto-Chemistry:- 1:100-1:800
Flow Cytometry:- 1:100-1:800
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.性能 标识 Cyanine Cy™3 保存方法 Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid for 1 year. 存储溶液 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
0.05% Sodium Azide浓度 1mg/ml