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- Rabbit anti-β-Actin Polyclonal Antibody
- Rabbit anti-β-Actin Polyclonal Antibody
概述 别名 β Actin抗体;Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin; beta; Beta actin; beta cytoskeletal actin; A X actin like protein; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN,betaActin宿主 Rabbit特异性 beta-Actin (Loading Control)反应种属 Human, Mouse, Rat, Hamster预测反应种属 Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat应用 WB: 1:5000-50000, IHC-F: 1:200-1000, IHC-P: 1:200-1000, IF: 1:200-1000, ICC/IF: 1:100-500, FCM: 1ug/Test分子量 Predicted molecular weight: 42kDa免疫原 Synthetic MAP peptide derived from human beta-Actin(1-200/375)性能 形式 Liquid浓度 1mg/mL纯化方法 affinity purified by Protein A类型 Polyclonal Antibody克隆号 同种型 IgG储存/保存方法 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.存储溶液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.标记 Unlabeled修饰 Unmodified靶标 背景说明 Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.组织特异性 Ubiquitously expressed in all eukaryotic cells.翻译后修饰 ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.细胞定位 细胞浆UniProt P60709研究领域 研究领域 Signal TransductionCytoskeleton / ECMCytoskeletonMicrofilamentsActin etcActinTags & Cell MarkersSubcellular MarkersCytoskeletonActin实验结果图Sample:Embryo Cerebrum (Mouse) Lysate at 40 ug Du145 (Human) Lysate at 40 ug SW480 (Human) Cell Lysate at 40 ug U87MG (Human) Lysate at 40 ug U251 (Human) Lysate at 40 ug A673 (Human) Lysate at 40 ug Lovo (Human) Lysate at 40 ug 293FT (Human) Lysate at 40 ug JEG-3 (Human) Lysate at 40 ug RSC96 (Rat) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 40 ug HepG2 (Human) Lysate at 40 ug A431 (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kDMCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Anti-beta-Actin at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kDBlank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody Dilution: 1ug/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1ug/T. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.温馨提示：本产品仅作科研实验使用，不支持临床等研究
