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- β-Actin抗体
- β-Actin抗体
概述 描述 β-Actin抗体, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.
别名 β-Actin抗体宿主 Rabbit特异性 Beta actin antibody detects endogenous levels of total Beta actin.反应种属 Human, Mouse, Rat, Pig, Zebrafish, Dog, Monkey, Fish预测反应种属 Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;应用 WB 1:3000-1:20000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000分子量 Predicted molecular weight: 42kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.免疫原 A synthesized peptide derived from human Beta actin.性能 浓度 1mg/ml纯化方法 The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .类型 Polyclonal Antibody储存/保存方法 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles存储溶液 Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.靶标 背景说明 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.翻译后修饰 ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
细胞定位 Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;UniProt P60709