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- Rabbit anti-KRAS Polyclonal Antibody

- Rabbit anti-KRAS Polyclonal Antibody
Rabbit anti-KRAS Polyclonal Antibody
概述 别名 KRAS抗体;NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; KRAS,KRAS宿主 Rabbit反应种属 Human, Mouse, Rat应用 WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.分子量 Detects a band of approximately 21kDa (Predicted molecular weight22kDa)免疫原 A synthetic peptide corresponding to a sequence within amino acids 30-189 of human KRAS (NP_203524.1).阳性对照 A-549,NIH/3T3,Raji,HeLa,Mouse spleen,Mouse brain,Mouse lung,Rat brain性能 形式 Liquid纯化方法 Affinity purification类型 Polyclonal Antibody同种型 IgG储存/保存方法 Store at -20℃. Avoid freeze / thaw cycles.存储溶液 PBS with 0.05% proclin300,50% glycerol,pH7.3.标记 Unlabeled修饰 Unmodified靶标 背景说明 This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.细胞定位 Cell membrane,Cytoplasm,Cytoplasmic side,Lipid-anchor,cytosolUniProt P01116实验结果图Western blot analysis of various lysates using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.Western blot analysis of lysates from C6 cells, using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.Western blot analysis of various lysates using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.Immunofluorescence analysis of HeLa cells using KRAS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 cells using KRAS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Rabbit anti-KRAS Polyclonal Antibody温馨提示:本产品仅作科研实验使用,不支持临床等研究





