DES1-3 IGF-1蛋白

Human

Escherichia coli.

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA

No Tag

> 97 % by SDS-PAGE and HPLC analyses.

Less than 50 EU/mg of Recombinant HumanDES1-3 IGF-1 as determined by LAL method.

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

P05019

3479

1. Ross M, Francis GL, Szabo L, et al. 1989. Biochem J, 258: 267-72.
2. Kummer A, Pulford BE, Ishii DN, et al. 2003. Int J Exp Diabesity Res, 4: 45-57.