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- Recombinant Bovine bFGF 10ug
- Recombinant Bovine bFGF 10ug
概述 描述 别名 种属 Bovine 形态 Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. 来源 Escherichia coli. 氨基酸序列 MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS 内毒素水平 Less than 1 EU/µg of rBobFGF as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107IU/mg. Accession # P03969 预期分子量 Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids. SDS-PAGE 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 97 % by SDS-PAGE and HPLC analyses. Gene IDs 281161 参考文献
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