＞98%

Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.

盐suan美他环素；盐酸甲烯tu霉素

黄色结晶性粉末

DMSO : 3 mg/mL (6.26 mM)

Water : 5 mg/mL (10.44 mM)

protein synthesis

Methacycline inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline inhibits TGF-β1-induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.

Methacycline (100 mg/kg, i.p.) delivered daily beginning 10 days after intratracheal bleomycin improves survival at Day 17. Methacycline attenuates Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA. Methacycline does not attenuate inflammatory cell accumulation or alter TGF-β1-responsive genes in alveolar macrophages.