The Prestained Protein Ladder/marker is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder/marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder/marker is supplied in gel loading buffer and is ready to use.
别名 蛋白marker；预染蛋白marker ；彩虹蛋白marker ;Protein Marker；Multicolor Protein Marker 形式 Approximay 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). 性能 QC Under suggested conditions, Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane. 保存方法 Stable for up to 2 weeks at 25°C; Stable for up to 3 months at 4°C ;For long term storage, store at -20°C 分子量信息 Migration patterns of Prestained Protein Ladder in different electrophoresis conditions are listed below: