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- Recombinant Human IFN-γ
- Recombinant Human IFN-γ
概述 种属 Human 形态 Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. 来源 Escherichia coli. 氨基酸序列 MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ 内毒素水平 Less than 1 EU/µg of Recombinant HumanIFN-γ as determined by LAL method. 活性 Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml. Accession # P01579 预期分子量 Approximay 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids. 性能 溶解方法 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 保存方法 This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 纯度 > 98 % by SDS-PAGE and HPLC analyses. Gene IDs 3458 参考文献 1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.<br/>3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.